eGene™ Constructs
Nuclera eGene constructs allow the user to screen the protein of interest alongside 7 solubility tags and no solubility tags option.
There is the option to work with Linear or Circular eGene constructs.
Linear eGene Constructs
The Linear eGene constructs have the following structure:
- The left megaprimer may contain a variety of solubility tags, while the right megaprimer always includes a GFP based detection tag (DET, 17 amino acids long, 1.95 kDa) along with a streptactin based purification tag (STREP).
- Importantly, the left megaprimer incorporates a 3C protease cleavage site at its 3’ terminus while the right megaprimer incorporates TEV protease site at the 5’ terminus providing the user flexibility to easily cleave off the additional tags from the purified proteins.
- The 3C-TEV protease cleavage sites also serve as the hybridization site in the overlap extension assembly reaction. Therefore, your gene of interest must be pre-adapted with 3C and TEV adaptor sequences at the 5’ and 3’ ends respectively, to be compatible with the eGene Prep Kit workflow.
To work with Linear eGene Construct it is possible to either prepare it through the eGene Prep Kit, or to order it through our eGene Service
Circular eGene Constructs
Alternatively to Linear eGene construct it is possible to use sequence-verified circular eGene™ constructs delivered as complete expression cassettes (see image below). This option it is available through our verified vendors GeneArt and GenScript
Which one to choose
The main differences and point of strength for the two different options are explained in the following table:
| DNA options | Custom Linear eGene™ | Gene fragments (dsDNA) | Circular eGene™ |
|---|---|---|---|
| Preparation | Ready-to-use. Prepared by our service team. | 5 hour PCR eGene™ Prep. | 30 minute enzyme incubation. |
| Lead time | 14–18 business days. | 10 business days. | Up to 6 weeks. |
| Kit required | None | Linear eGene™ Prep Kit Solubility or FlexiVariant™ screen | Circular eGene™ Prep Kit. |
Nuclera Technical Support:
UK Phone +44 1223 942 761
US Phone: +1 508-306-1297
Email: techsupport@nuclera.com
Offices:
Nuclera UK (HQ):
One Vision Park, Station Road, Cambridge, CB24 9NP, UK
Nuclera USA: 1000 Technology Park Drive, Suite B, Billerica MA 01821, USA www.nuclera.com
Copyright © 2025 Nuclera Ltd. All trademarks are the property of Nuclera, Ltd. Visit nuclera.com/legal for more info.