16 docs tagged with "eProtein Discovery"

This protocol provides general guidelines to clone linear eGene™ constructs into an expression vector

This protocol describes the process of biotinylating AviTag™-labelled proteins produced with eProtein Discovery™ using biotin ligase (BirA). The AviTag™ sequence must be introduced into the gene-of-interest, located between the 3C and TEV adaptor sequences.

The eProtein Discovery instrument will show, at the end of the experiment, the four best obtainable combinations of eGene™ construct and Cell-free Blend.

eProtein Discovery Results

The design of the experiment can be carried out on the Cloud Software or using the provided spreadsheet for the standalone system

The design of the experiment can be carried out on the Cloud Software ore usign the provided spreadsheet for the standalone system

Nuclera eProtein Discovery™ System is the only end-to-end protein prototyping system that accelerates construct design, expression, solubility characterization and purification of target proteins in drug discovery programs. Accelerating the journey to your protein.

After proceeding to the next step of the checklist, the experiment is initiated and it is not possible to go back and forth on the screen.

After proceeding to the next step of the checklist, the experiment is initate and it is not possible to go back and forth in the screen.

When ordering linear DNA follow the steps outlined below.

Before you begin preparing your reagents, please ensure the Base Fluid has been connected to the instrument overnight.

Scale-up expression and purification

Connect the vial of base fluid to the pump module

Connect the vial of base fluid to the pump module

Single Sign On

Introduction