Prepare the transfer plate

Check that the base fluid has been equilibrated

Before you begin preparing your reagents, please ensure the Base Fluid has been connected to the instrument overnight. If the Base Fluid was not connected in advance, please open a new vial and incubate it for at least 1 hour at 30°C before use

Take all the reagents out of the freezer. The preparation of the transfer plate takes about 1 hour.

Our Quick Start User Guide can be used as a short guide tool how to prepare the transfer plate

The eProtein Discovery™ reagents need to be prepared and loaded onto a 96 well transfer plate following the layout and volumes in Figure 5 and Table 2.

It is critical to follow this layout exactly because it determines how the reagents are dispensed in the eProtein Discovery™ cartridge.

If an eGene™ construct is missing it must be substituted with 5 µL of Blank Buffer.

Important Note

Do not substitute a missing eGene™ construct with water as this can have a significant negative impact on the droplet operations on the eProtein Discovery™ cartridge

Figure 5: Transfer plate layout

eGene™ DNA construct

Reagent	Well	Volume (µL)
eGene™ construct	A1 - A8	5
eGene™ construct	B1 - B8	5
eGene™ construct	C1 - C8	5

Reagents from NC3010

Reagent	Well	Volume (µL)
Elution Buffer	H1-H2	10
Blank Buffer	H3 - H4	10
Universal Control	H5	10
Complemantion Control	H6	10
Workflow Control	H7	10
Expression Control	H8	10
Detector Protein	A10, B10, G10, H10	16
Wash Buffer	C10, D10	16
Elution Buffer	G10	16
Cell-free Core (16 µL) + Additive 1 (2 µL) + Additive 2 (2 µL )	12A - 12H	20 (16+2+2)

Reagent	Volume (µL)
eGene™ construct	5
Controls: Blank Buffer, Universal Control (Univ. Ctrl), Complementation control (Comp. Ctrl), Full Workflow Control (W/F), Expression Control (Exp Ctrl)	10
Cell-free Blend (CFB): Cell-free Core Reagent + Additive 1 + Additive 2	20 (16+2+2)
Wash Buffer (Wash Buffer)	16
Elution Buffer (Elut. Buffer)	10 µL in H1 & H2, 16 µL in F10
Detector Protein (Det. Prot.)	16