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Transfer Plate and Beads Preparation Quick Start

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Transfer plate​

IMPORTANT NOTE

Keep the transfer plate and the reagents from Nuclera branded box with a yellow label on ice

Strep Purification Beads​

IMPORTANT NOTE

Strep Purification Beads are provided in 200 μL aliquots of 5% v/v suspension and must be stored at +4°C.

  1. Take the vial of Strep Beads from the fridge and give it a quick spin for 2 seconds in a microcentrifuge to pellet the beads.
  2. Resuspend the beads by gently pipetting up and down 10 times with a p200 pipette set on 90 μL.
  3. Transfer 90μL of the resuspended beads into a 1.5 mL tube.
  4. Place the tube with Strep Beads on a magnetic rack and capture for 1 min.
  5. Remove all the supernatant with a p200 pipette and discard the liquid.
  6. Remove the tube with Strep Beads from the magnetic rack. Resuspend the beads in 100 μL Wash Buffer by slowly pipetting up and down 10 times.
  7. Repeat steps 4 to 6 twice more for a total of three washes.
  8. After the third wash, spin down the tube and place it back on a magnetic rack and capture for 1 min.
  9. Remove all the supernatant with a p200 pipette and discard the liquid.
  10. Spin down the tube, place it back on a magnetic rack and remove the residual buffer with a p10 pipette.
  11. With a p20 pipette, resuspend the beads in 10.5 μL Wash Buffer by gently pipetting up and down 10 times to create a 15 μL 30% Strep Beads working dilution.
  12. Keep the beads in the tube on the bench, not on ice. The beads should not be loaded onto the transfer plate.