Guidelines to prepare SDS samples

Prepare samples for SDS page gel

This section aims to give a guideline to run a commercial 15 combs SDS page gel.

For the SDS page gel you will need 4 tubes

	Sample	Lab Grade Water	3.6x Loading reducing dye
Eluted Protein	3 µL	5.7 µL	3.3 µL
Core + NFW (negative control)	3 µL	19 µL	9 µL
Crude CFPS	3 µL	19 µL	9 µL

Standards
To have a semiquantitative assay, you can prepare and run BSA standards alongside your samples.
Dilute your BSA sample to 1 mg/mL and make dilution as outlined in the table below to prepare your 3 standards (A, B and C)

	Required Stocl	Lab Grade Water	3.6x Loading reducing dye
Standard A	BSA 1 mg/mL	24 µL	176 µL
Standard B	Standard A	50 µL	150 µL
Standard C	Standard B	50 µL	150 µL

Load the SDS PAGE gel
Load on the stain-free protein gel, 4-15% (15 well):

4 µL pre-stained protein ladder
4 µL eluted scaled-up protein sample
8 µL eluted scaled-up protein sample
4 µL No-DNA negative expression control
4 µL positive expression control
4 µL BSA standard A
4 µL BSA standard B
4 µL BSA standard C

Run at 200 V for 40 minutes

Nuclera Technical Support:
UK Phone +44 1223 942 761
US Phone: +1 508-306-1297
Email: techsupport@nuclera.com

Offices:
Nuclera UK (HQ):
One Vision Park, Station Road, Cambridge, CB24 9NP, UK

Nuclera USA:
1000 Technology Park Drive, Suite B, Billerica MA 01821, USA
www.nuclera.com

Prepare samples for SDS page gel​

Prepare samples for SDS page gel